Effects of picloram and other auxins on onion tissue cultures
1983
A:PS
Details
Title
Effects of picloram and other auxins on onion tissue cultures
Author
Phillips, G.C.
Luteyn, K.J.
Luteyn, K.J.
Publication Date
1983
Call Number
A:PS
Summary
Seedling shoot meristem-tip expants of `Yellow Grano` and `Yellow Sweet Spanish` onions (Allium cepa L.) were cultured in vitro on media varying in auxin and cytokinin composition, 4-Amino-3,5,6-trichloropicolinic acid (picloram) as an auxin source was superior to alpha-naphthaleneacetic acid (NAA) used as a contorl for shoot multipliction cultures maintained for 6 months or longer. a combination of 0.03 mg/liter picloram and 0.5 mg/liter 6-benzylamino purine (BA) was optimal. Picloram was comparable or superior to the 2,4-dichlorophenoxyacetic acid (2,4-D) control for induction, continued maintenance and friability of callus, and subsequent regeneration of plants. Somatic embryos developed at high frequency in some lines of both cultivars on media containing 0.75 mg/liter picloram and 1.5 and 2.0 mg/liter BA. The most promising scheme for plant regeneration from 6 to 12-month-old callus cultures involved transfer from somatic embryo induction media shoot multipliction media. These techniques also were successful using mature bulb basal plate-scale explants. [AS]
Journal Citation
v.108(6):948-953, AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles