Solanaceas crops infected by wide range of diseases one of the most important disease is bacterial wilt caused by Ralstonia solanacearum species complex one of the most destructive phytopathogenic bacteria. It enters into plant natural openings and wounds caused by implements& insect pest. Ralstonia solanacearum is a soil born pathogen having special features like wide host range, latent infection, survives in soil up to 20 years. So that the disease management is more difficult. Proper identification, distribution and mapping plays an important role in the development of effective management strategies. Wilted tomato, sweet pepper, hot pepper, African eggplant and African night shade plant samples are collected from different regions of Tanzania. The causal bacterial strain were isolated from the samples and all were identified as Ralstonia solanacearum. Multiplex PCR using phylotype specific primer pair identified the presence of Phylotype I strain is the most common then Phylotype III and Both Phylotype I & Phylotype III strains are present in same field /plant. Phylotype I is widely distributed in Tanzania fields whereas Phylotype III is confined to Kilimanjaro and Dodoma region both Phylotype I & Phylotype III are present in fields of Morogoro, Dodoma , and Arusha regions of Tanzania.