000075828 001__ 75828
000075828 005__ 20230202174449.0
000075828 0247_ $$2doi$$a10.1094/PHYTO-09-22-0355-R
000075828 037__ $$aRESEARCH$$bPublished Articles
000075828 041__ $$aeng
000075828 090__ $$aE15164
000075828 245__ $$aMolecular epidemiology of Ralstonia pseudosolanacearum phylotype I strains in the South West Indian Ocean region and their relatedness to African strains
000075828 269__ $$a2022
000075828 336__ $$aPublished Articles
000075828 520__ $$aIncreasing requirements for developing tools allowing fine strain traceability responsible for epidemics is tightly linked with the need to understand factors shaping pathogen populations and their environmental interactions. Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is one of the most important plant diseases (Mansfield et al., 2012) in tropical and sub-tropical regions. Sadly little or outdated, or no information on its epidemiology is reported in the literature, although alarming outbreaks are regularly reported as a disaster. A large set of phylotype I isolates (n = 2,608) was retrieved from diseased plants in fields across the South West Indian Ocean (SWIO) and Africa. This collection enabled further assessment of the epidemiological discriminating power of the previously published RS1-MLVA14 scheme. Thirteen markers were validated and characterized as not equally informative. Most had little infra-sequevar polymorphism and their performance depended on the sequevar. Strong correlation was found with a previously MLST scheme. However, 2-3% of sequevars were not correctly assigned through endoglucanase gene sequence. Discriminant Analysis of Principal Components (DAPC) revealed four groups with strong phylogenetic relatedness to sequevars 31, 33, and 18. Phylotype I-31 isolates were highly prevalent in the SWIO and Africa, but their dissemination pathways remain unclear. Tanzania and Mauritius showed the greatest diversity of RSSC strains, as the four DAPC groups were retrieved. Mauritius was the sole territory harboring a vast Phylogenetic diversity and all DAPC groups. More research is still needed to understand the high prevalence of phylotype I-31 at such a large geographic scale.
000075828 590__ $$aNew article
000075828 650__ $$059218$$aBACTERIAL WILT
000075828 650__ $$059212$$aPLANT DISEASES
000075828 691__ $$aC050
000075828 7001_ $$aCellier, Gilles
000075828 7001_ $$aNordey, Thibault
000075828 7001_ $$aCortada, Laura ... et al.
000075828 773__ $$jonline$$tPHYTOPATHOLOGY
000075828 8564_ $$9e837aeab-57f3-408f-a83a-692fb20eaafe$$s1631072$$uhttps://worldveg.tind.io/record/75828/files/e15164.pdf
000075828 980__ $$aLIB