Bacterial wilt in tomato plants is caused by Ralstonia solanacearum species complex. Tomato crops has economic significance, hence the loss of the disease is a huge one. Fortunately, it can be managed by resistant cultivars, which is promised to be an effective and environment friendly approach. For the screening of resistant cultivars, traditional screening processes can take a long time, but recently, published research found out a rapid screening method is applicable for tomato plants. Therefore, we aim to establish the cotyledon-screening method as a rapid and reliable alternative compare to traditional method. The method was undecided that how many days after inoculation is most applicable for measuring their reaction, hence we took wilting percentage from 5, 7 and 10 days after inoculation and select which set of data correlate reliable to the data from the traditional screening. The results showed that the 10th day after inoculation has the highest correlation coefficient. From carrying out the trials, we found out the advantages of the method as well as data that suggested when the method is appropriate and applicable. This experiment results provide us a preliminary cotyledon-screening method, more details will need further improvement through more modified experiment in the future.