000074512 001__ 74512 000074512 005__ 20220124154141.0 000074512 0247_ $$2doi$$a10.3389/fpls.2021.798858 000074512 037__ $$aRESEARCH$$bStaff Publications 000074512 041__ $$aeng 000074512 088__ $$aWorldVeg Staff Publication 000074512 090__ $$aE14974 000074512 245__ $$aRNA-Seq transcriptome analysis provides candidate genes for resistance to tomato leaf curl New Delhi Virus in melon 000074512 269__ $$a2022 000074512 336__ $$aStaff Publications 000074512 520__ $$aTomato leaf curl New Delhi virus (ToLCNDV) emerged in the Mediterranean Basin in 2012 as the first DNA bipartite begomovirus (Geminiviridae family), causing severe yield and economic losses in cucurbit crops. A major resistance locus was identified in the wild melon accession WM-7 (Cucumis melo kachri group), but the mechanisms involved in the resistant response remained unknown. In this work, we used RNA-sequencing to identify disease-associated genes that are differentially expressed in the course of ToLCNDV infection and could contribute to resistance. Transcriptomes of the resistant WM-7 genotype and the susceptible cultivar Piñonet Piel de Sapo (PS) (C. melo ibericus group) in ToLCNDV and mock inoculated plants were compared at four time points during infection (0, 3, 6, and 12 days post inoculation). Different gene expression patterns were observed over time in the resistant and susceptible genotypes in comparison to their respective controls. Differentially expressed genes (DEGs) in ToLCNDV-infected plants were classified using gene ontology (GO) terms, and genes of the categories transcription, DNA replication, and helicase activity were downregulated in WM-7 but upregulated in PS, suggesting that reduced activity of these functions reduces ToLCNDV replication and intercellular spread and thereby contributes to resistance. DEGs involved in the jasmonic acid signaling pathway, photosynthesis, RNA silencing, transmembrane, and sugar transporters entail adverse consequences for systemic infection in the resistant genotype, and lead to susceptibility in PS. The expression levels of selected candidate genes were validated by qRT-PCR to corroborate their differential expression upon ToLCNDV infection in resistant and susceptible melon. Furthermore, single nucleotide polymorphism (SNPs) with an effect on structural functionality of DEGs linked to the main QTLs for ToLCNDV resistance have been identified. The obtained results pinpoint cellular functions and candidate genes that are differentially expressed in a resistant and susceptible melon line in response to ToLCNDV, an information of great relevance for breeding ToLCNDV-resistant melon cultivars. 000074512 650__ $$057922$$aCUCUMIS MELO 000074512 650__ $$059322$$aRESISTANCE 000074512 6531_ $$aMELONS 000074512 6531_ $$aTOMATO LEAF CURL NEW DELHI VIRUS 000074512 6531_ $$aTRANSCRIPTOME (RNA-seq) 000074512 6531_ $$aDEG (DIFFERENTIALLY EXPRESSED GENES) 000074512 6531_ $$aqPCR (QUANTITATIVE POLYMERASE CHAIN REACTION) 000074512 691__ $$aB370 000074512 7001_ $$aSáez, Cristina 000074512 7001_ $$aFlores-León, Alejandro 000074512 7001_ $$aMontero-Pau, Javier 000074512 7001_ $$aSifres, Alicia 000074512 7001_ $$aDhillon, Narinder P. S. 000074512 7001_ $$aLópez, Carmelo 000074512 7001_ $$aPicó, Belén 000074512 773__ $$j12:798858$$tFRONTIERS IN PLANT SCIENCE 000074512 8564_ $$94dcac411-521f-42e7-8aeb-ad7cdeda0e6f$$s10416757$$uhttps://worldveg.tind.io/record/74512/files/e14974.pdf 000074512 980__ $$aMIG