Eggplant (Solanum melongena) is one of vegetables that has high economic value. Eggplant is widely cultivated throughout the world in both tropical and sub-tropical countries. One of the diseases that attack eggplant is bacterial wilt caused by the bacterium Ralstonia solanacearum. Bacterial wilt is one of the most damaging diseases for eggplant. The most effective and efficient way to overcome bacterial wilt is to use resistant cultivars. Bacterial wilt resistance is highly affected by environmental factors, especially temperature factor. At high temperatures, often resistant cultivars become susceptible. Therefore eggplant cultivars that has good resistance to bacterial wilt are needed both in normal and high temperature conditions. In this study, 40 S2 eggplant lines of 5 different genotypes were assessed for their resistance to bacterial wilt at conditions of 25C and 30C. Two strains originating from genotype VI041984, namely line no. 9 and 14 have good resistance to bacterial wilt under conditions of 25C and 30C. From the results of the latent infection test and the measurement of bacterial concentration, it was found in these two strains, Ralstonia solanacearum was able to infect and reproduce well but was unable to cause symptoms of disease.