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Effects of exogenous nitric oxide on growth and transcriptional expression of antioxidant enzyme mRNA in tomato seedlings under copper stress
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Heavy metal stress badly affect the crop growth and development, assuaging heavy metal stress to crop show important for crop yield. Here we investigated the effect of SNP (sodium nitropprusside, an exogenous nitric oxide donor) on alleviating copper stress and regulating antioxidant gene transcriptional level in liquid-culture tomato seedlings. Our results showed that copper at 0.5 mmol . L -1 significantly suppressed the tomato seedlings growth including plant height, stem thickness, plant fresh weight and plant dry weight, as well as improved the transcriptional levels of antioxidant genes encoding peroxidase (POD) , ascorbate peroxidase (APX), superoxide dismutase (SOD) and catalase ( CAT). Exogenous application of O. 3 mmol . L-1 SNP remarkably alleviated copper-inhibition to tomato growth and further enhanced these antioxidant genes transcription, however, additional L-NAME (NO) -nitro-L-arginine methyl ester) , the special inhibitor of nitric oxide synthase (NOS) obviously aggravated copper-induced inhibitory effect on tomato growth and reduced antioxidant enzyme gene transcriptional level. Basing on these results we propose that tomato NOS enzyme-mediated NO is necessary for tomato responding to copper stress and NO application lessen copper stress to tomato growth via enhancing antioxidant enzyme gene transcriptional levels.
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 Record created 2010-04-20, last modified 2019-07-02

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