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Novel application of H tube electrophoresis to separation technology
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The H tube electrophoresis technique was used for isolating human IgG by the net effect of electrophoresis and electro-endosmosis in agarose gel containing human serum. In asimilar experiment human serum albumin was purified by extracting the agarose gel slice containing the albumin following zone electrophoresis of serum in agarose. By taking advantage of the relatively high reverse electro-endosmotic flow compared to the migration of IgG in agarose gel, sheep anti chicken immunoglobul in was fractionated into active and inactive portions. It was estimated that 90% of the nonspecific IgG could be el iminated in this manner. The specific immunoglobul in fraction showed a higher mobil ity to the positive electrode than the bulk of the non specific IgG. The H tube proved to be ideal for zone electrophoresis in concentration gradients of inert substances. For this purpose sugar gradients were used for electrophoretic separation of proteins and viruses which are not affected by high osmotic pressures but, for separating animal cells it was necessary to use Ficoll gradients. A mixture of ye11ow Atypical Tomato Mosaic Virus and the haemocyanin of Burnupena cincta were separated into discrete zones and chicken IgY anti haemocyanin was subfractionated. The specific fraction showed a lower migration rate to the positivem pole than the bulk of the non specific material.
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 Record created 2017-10-01, last modified 2018-02-14

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