Record Details

Carbohydrate composition and electrophoretic properties of tomato polygalacturonase isoenzymes
Publication date:
Two polygalacturonase isoenzymes, PG I and PG II, were extracted from Murrieta tomato and purified by gel exclusion and ion-exchange chromatography. The kinetic constants and activation energies of the purified isoenzymes have been determined. Polygalacturonase I has two polypeptide chains (Mr=47500 and 41400) whereas polygalacturonase II is a single polypeptide (Mr=47500) as shown by electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulphate. Both isoenzymes are glycoproteins. Through gas liquid chromatography, polygalacturonase II was shown to contain 4.6% neutral hexoses and 1.5% amino sugars. There are eight D-mannose, two L-fucose, two D-xylose and three N-acetylglucosamine residues per mole of PG II. The carbohydrate portion of PG II was shown to be atacched to the protein part through an N-acetylglucosaminylasparaginyl bond. [AS]
Call number:
Journal citation:
Contact information:

Record appears in:


 Record created 2017-10-01, last modified 2019-02-22

Rate this document:

Rate this document:
(Not yet reviewed)