Reduction in the in vitro germination of conidia of Alternaria alternata isolated from Eruca sativa with juice of broccoli
2013
E09866
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Title
Reduction in the in vitro germination of conidia of Alternaria alternata isolated from Eruca sativa with juice of broccoli
Author
Flores Cordova, M. A.
Martinez Damian, M. T.
Nieto Angel, D.
Rodriguez Perez, J. E.
Colinas Leon, M. T.
Martinez Solis, J.
Martinez Damian, M. T.
Nieto Angel, D.
Rodriguez Perez, J. E.
Colinas Leon, M. T.
Martinez Solis, J.
Publication Date
2013
Call Number
E09866
Summary
The fungus Alternaria alternata, is considered one of the major plant pathogens worldwide causing the symptom of leaf spot in vegetables. Glucosinolates are natural products derived from broccoli, containing nitrogen and sulfur, with antifungal activity. Based on the above, the present study aimed to evaluate the effectiveness of broccoli juice in vitro on germination of conidia of A. alternata. To carry out the experiment, leaves of Eruca sativa (arugula) with symptoms of leaf spot were collected, from which the fungus A. alternata was isolated and identified. Conidia of the fungus were placed on PDA medium with different concentrations of glucosinolates found in broccoli juice 0.15, 0.11, 0.07, 0.04, 0.01, and 0 ng/uL. It was observed that the concentrations 0.07, 0.1, 0.15 ug/uL inhibited 100% spore germination of A. alternata at 6 h evaluation. The average lethal concentration LC was 0.028 ug/uL. In 50 conclusion, it can be mentioned that the glucosinolates present in broccoli juice, have an antifungal potential and can be used as post-harvest treatment, in order to reduce the damage caused by A. alternata in vegetables.
Journal Citation
31(2):180-190, REVISTA MEXICANA DE FITOPATOLOGIA
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles