Screening method for resistance to pepper fruit anthracnose: pathogen sporulation, inoculation methods related to inoculum concentrations and post-inoculation environment
2001
Details
Title
Screening method for resistance to pepper fruit anthracnose: pathogen sporulation, inoculation methods related to inoculum concentrations and post-inoculation environment
Author
Yoon, J.B.
Park, H.G.
Park, H.G.
Publication Date
2001
Summary
Pepper (Capsicum spp.) anthracnose caused by Colletotrichum gloeosporioides is the most destructive disease reducing marketable fruit yields and decreasing fruit quality of pepper in Korea Republic. This experiment as a fundamental research for resistance breeding was conducted to establish a more reliable screening method based on sporulation of C. gloeosporioides [Glomerella cingulata] for inoculum preparation, and inoculation methods combining inoculum density and control of post-inoculation environment for testing resistance. Sporulation of C. gloeosporioides was most abundant on PDA (potato dextrose agar) medium under 30 degrees C and 16 h illumination. In inoculation methods combined with inoculum concentrations, microinjection as a wound-inoculation method was more repeatable and distinguishable on symptom development under all inoculum concentrations as well as expressed disease symptoms under the lowest inoculum density. Also, microinjection was more repeatable and consistent compared with pinning as a different wound-inoculation method on symptom development and precision of resistance evaluations. This result was, maybe, due to a uniformly adjustable ability to wounding depth in microinjection method. The 30 degrees C of post-inoculation temperature was optimal condition and the post-inoculation light treatment did not influence on symptom development.
Journal Citation
42(4):389-393, KOREAN SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles