Determination of incidence of seed borne fungi on mungbean (Vigna radiata (L.) Wilczek)

Determination of incidence of seed borne fungi on mungbean (Vigna radiata (L.) Wilczek)

2013

E07321

Good quality seed is the prerequisite to agriculture and though of such vital significance very less attention has been paid to their efficient storage and storage losses. The present study was undertaken to screen for isolation and percent incidence of seed borne mycoflora where two mungbean varieties viz. HUM-4 and HUM-12 were screened by standard blotter paper and agar plate methods. Seeds of mungbean varieties were treated with 2.5% bleach-NaOCl2 for 1 minute and washed thoroughly with distilled water. Only sterilized seed were used for detection of seed borne pathogen. Agar plate method was found to be suitable as even under lesser incubation there was higher observed percent incidence of seed mycoflora. Altogether 17 different fungi were found associated with the seed samples. Aspergillus flaws, Aspergillus niger, Aspergillus terreus, Alternaria sp., Curvularia sp., Fusarium sp., Macrophomina phaseolina and Penicillium sp. were predominant in standard blotter paper and agar plate methods but in percent incidence of seed mycoflora were found to be more abundant in agar plates as compared to blotter methods. Aspergillus niger and Macrophomina phaseolina were the predominant mycoflora associated with HUM-4. Highest germination was recorded in Mungbean variety HUM-12 in blotter method and lowest in variety HUM-4 in agar plate method.

31(2):564-568, ENVIRONMENT AND ECOLOGY

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