000049195 001__ 49195 000049195 005__ 20190704163338.0 000049195 035__ $$a(wvc)59460 000049195 037__ $$aRESEARCH$$bPublished Articles 000049195 041__ $$aeng 000049195 090__ $$aE07084 000049195 245__ $$aEnhancement of catalase activity under salt stress in germinating seeds of Vigna radiata 000049195 269__ $$a2013 000049195 336__ $$aPublished Articles 000049195 520__ $$aCatalase(CAT) (EC 1.11.1.6) is an important cellular antioxidant enzyme that defends against oxidative stress. Hydrogen peroxide, although not a free radical, is highly reactive. It serves to protect the cell from toxic effects of high concentrations of hydrogen peroxide by catalyzing its decomposition into molecular oxygen and water, without the production of free radicals. The effects of presoaking of seeds in salt solution on germination and catalase activity of Vigna radiata were studied. NaCl and CaCl2soaked seeds showed reduced germination (less than 1cm) as indicated by decreased shoot length when compared to controls. 5g seeds of Vigna radiata were presoaked for 24 hours in presence of NaCl (1, 5, 10, 15, 20 M) and CaCl2 (0.5, 1, 1.5, 2.0 M). After two days of germination, the filtered water extracts (25 ml) were tested for qualitative catalase activity. Further catalase assay of respective extracts was monitored in terms of decay in H2O2 concentration at 240 nm (Jasco-V-530) and compared with the decay in the absence of stress. There was nearly 40% increase in extent of decay of H2O2at concentration of 1M NaCl and 33.9% increase in decay at 1M concentration of CaCl2. Further study includes different combinations of NaCl and CaCl2to be tested followed by increase in germination period and catalase activity monitoring in partially purified and dialyzed extracts. There was an enhancement of catalase activity in presence of the salt stress. 000049195 590__ $$aMB-56 000049195 650__ $$aVIGNA RADIATA 000049195 650__ $$aSALT STRESS 000049195 650__ $$aCATALASES 000049195 650__ $$aGERMINATION 000049195 650__ $$aSEEDS 000049195 650__ $$aOXYGEN 000049195 650__ $$aENZYMES 000049195 691__ $$aB110 000049195 7001_ $$aSaraf, N. 000049195 773__ $$tASIAN JOURNAL OF BIOMEDICAL AND PHARMACEUTICAL SCIENCES$$j3(17):6-8$$yAJBPSH$$w49196 000049195 8564_ $$s447191$$uhttp://worldveg.tind.io/record/49195/files/e07084.pdf 000049195 901__ $$a64215 000049195 902__ $$aAV-13 000049195 903__ $$aN 000049195 903__ $$aZ 000049195 904__ $$aM 000049195 911__ $$aMungbean$$bMB$$cMAIN$$dMB-56 000049195 912__ $$e2013-05-10$$kCHEN 000049195 918__ $$aEn 000049195 980__ $$aLIB