Immunochemical characterization and localization of nitrate reductase in norflurazon-treated soybean cotyledons
1984
A:PS
Details
Title
Immunochemical characterization and localization of nitrate reductase in norflurazon-treated soybean cotyledons
Publication Date
1984
Call Number
A:PS
Summary
Immunochemical procedures were used to characterize and localize NADH:nitrate reductase (NR; EC 1.6.6.1) in cotyledons of norflurazon-treated soybeans [Glycine max (L.) Merr. cv. Hill]. Antiserum prepared to NR isolated from Chlorella strongly reacted against NR from norflurazon-treated cotyledons. This serum inhibited the NR activity in crude extracts of norflurazon-treated soybean cotyledons by 98% even at a 1:2000 dilution of crude serum. Pre-immune serum had no effect on the activity. These data indicate that there are similar antigenic determinants at the active site of both Chlorella and norflurazon-treated soybean NR. Whole cotyledons were homogenized in lithium dodecyl sulfate-containing buffer, electrophoretically separated and blotted to nitrocellulose. When the blots were reacted with the anti-NR serum only a single protein (Mr = 98 kdalton) was visualized. Immunofluorescence studies on fixed tissue sections revealed intense fluorescence in the cytoplasm. Weaker reactions were associated with organelles tentatively identified as plastids. Pre-immune serum controls were completely unstained using immunocytochemical procedures. [AS/SS]
Journal Citation
v.62(3):481-484, PHYSIOLOGIA PLANTARUM
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles