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Attachment of N-acetyl-L-methionine into whole soybeans and the nutritional consequences for the rat
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The reactive N-hydroxysuccinimide ester of N-acetyl-L-methionine was used to attach N-acetyl-L-methionine (NAM) into whole soybeans. The modified soybeans contained 70% more methionine than the original soybeans. The added methionine was presumably present in the modified soybeans as NAM covalently attached to protein amino groups or other nucleophilic groups or tightly adsorbed by some noncovalent linkage since it was not removed by exhaustive dialysis. Rat feeding experiments using 10% protein diets demonstrated that NAM in the modified soybeans was biologically available, although to a lesser extent than free DL-methionine added to unmodified soybeans. The unadjusted protein efficiency ratio (PER) value of modified soybeans was 1.75+-0.10 compared to 1.19+-0.08 of control soybeans. When control soybeans were supplemented with free DL-methionine to equal the level of total methionine of modified soybeans, the PER value was increased to 2.29+-0.05. [AS/SS]
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 Record created 1985-05-31, last modified 2019-03-05

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