Characterization of polyphenol oxidase from susceptible and resistant varieties of tomato inoculated with the root knot nematode, Meloidogyne incognita
1982
REP.TM-0544
Details
Title
Characterization of polyphenol oxidase from susceptible and resistant varieties of tomato inoculated with the root knot nematode, Meloidogyne incognita
Author
Ganguly, S.
Dasgupta, D.R.
Dasgupta, D.R.
Publication Date
1982
Call Number
REP.TM-0544
Summary
Partially purified preparations of polyphenol oxidase from nematode-inoculated susceptible (Pusa Ruby) and resistant (SL-120) tomato varieties were subjected to the enzyme-characterization studies. The results revealed that polyphenol oxidase components from all the samples were heat stable and denatured after an exposure of the enzyme to 40 degree C for half an hour. The enzyme remained active at wide range of pH(3-8) with 2-3 pH optima. The enzyme from resistant variety was found to be more stable as compared to that of susceptible variety. It was apparent from this investigation that mercaptoethanol, sodium bisulphite, glutathione, sodium azide and certain phenolic acids were found to be strong inhibitors of enzyme. The conclusive evidences showed the differences in the enzyme characters for Pusa Ruby and SL-120, which indicate that the enzyme components of susceptible variety were different from that of resistant variety. [AS]
Journal Citation
v.12(2):320-329, INDIAN JOURNAL OF NEMATOLOGY
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles