Record Details

Title:
An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2
Publication date:
1989
Summary:
The locus, I2, which in tomato confers resistance against F. oxysporum f.sp. lycopersici race 2, was introgressed into Lycopersicon esculentum from the wild species L. pimpinellifolium (PI 126915). Since I2 maps to chromosome 11, DNA clones from this chromosome were used as hybridization probes to Southern blots containing bound DNA of nearly isogenic lines digested with 23 restriction enzymes. Of the 14 chromosome 11 clones, 9 exhibited polymorphism. These clones were further hybridized to verification filters that contained DNA from resistant and susceptible L. esculentum varieties digested with the enzymes that gave the polymorphism. One clone, TG105, was associated with I2; 19 susceptible lines showed a different RFLP with this probe than 16 resistant lines, including the original L. pimpinellifolium accession. These results were taken to indicate that TG105 is closely linked to the resistance gene.
Call number:
REP.TM-1442
Journal citation:
Linked resources:
Fulltext: d015566 - PDF
Contact information:
harvest@worldveg.org

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 Record created 2008-03-20, last modified 2019-03-11

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