Diversity of Ralstonia solanacearum infecting eggplant in the Philippines
2007
A:PS
Details
Title
Diversity of Ralstonia solanacearum infecting eggplant in the Philippines
Publication Date
2007
Call Number
A:PS
Summary
The diversity of Ralstonia solanacearum strains isolated from eggplant (Solanum melongena) grown in five provinces of the Philippine island group of Luzon was assessed using a recently described hierarchical system. All strains keyed to race 1, biovar 3 or 4. Phylotype-specific multiplex polymerase chain reaction (PCR) indicated that, like most other strains of Asian origin, all the strains in our Philippine collection belong to phylotype 1. Taxometric and phylogenetic analyses of partial endo\-glucanase gene sequences of strains from this collection and those previ\-ously deposited into GenBank revealed at least four subgroups among the otherwise monophyletic phylotype I strains. Nucleotide polymorphisms within each subgroup were infrequent and, among the subgroups identified in this study, variation was always <1.3%, indicating that the large majority of strains could be assigned to a single sequevar. Genomic DNA fingerplinting using enterobacterial repetitive intergenic consensus (ERIC)\-PCR revealed additional fine-scale genetic variation that was consistent with the endogluconase sequence data. Whole-pattern and band-based analyses of the genomic fingerprint data revealed four and eight distinct genotypes, respectively, within our collection. Eggplant from infested fields in different provinces tended to harbor mixed populations of ERIC genotypes, with the predominant genotype varying by location.
Journal Citation
v.97(11):1467-1475, PHYTOPATHOLOGY
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles